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· When adding or replacing medium, never touch the neck of the culture flasks with the bottle containing the medium or use the same pipette to transfer medium to more than one bottle. - Because of the risks of contamination and cross-infection, cell culture in the virus diagnostic laboratory is best carried out in closed vessels, usually screw-capped tubes and flat-sided bottles. - · Use 7-X, DECON or similar detergent for thorough cleaning of all laboratory glassware.
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